Wet-spinning of recombinant silk-elastin-like protein polymer fibers with high tensile strength and high deformability

Weiguo Qiu, Weibing Teng, Joseph Cappello, Xiaoyi Wu

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

A recombinant silk-elastin-like protein copolymer SELP-47K containing tandemly repeated amino acid sequence blocks from silk, GAGAGS, and elastin, GVGVP, was fabricated into microdiameter fibers using a wet-spinning technique. Raman spectral analysis revealed the formation of antiparallel β-sheet crystals of the silk-like blocks. Dry SELP-47K fibers display the dependence of mechanical properties such as Young's modulus on fiber diameter, suggesting more oriented and crystallized molecular chains in small-diameter fibers. Additionally, a brittle fracture mode was identified for dry fibers by SEM analysis of fracture surfaces. Hydration dramatically influenced the mechanical behavior of SELP-47K fibers. In contrast to the high tensile strength and limited strains to failure of dry fibers, fully hydrated SELP-47K fibers possessed strains to failure as high as 700%. Furthermore, upon chemical cross-linking, a tensile mechanical strength up to 20 MPa was achieved in hydrated fibers without compromising their high deformability. By combing the silk- and elastin-derived sequences into a single SELP-47K protein polymer, we demonstrated that protein fibers with high tensile strength and high deformability can be fabricated.

Original languageEnglish (US)
Pages (from-to)602-608
Number of pages7
JournalBiomacromolecules
Volume10
Issue number3
DOIs
StatePublished - Mar 9 2009

Fingerprint

Elastin
Spinning (fibers)
Silk
Tensile Strength
Formability
Polymers
Tensile strength
Proteins
Fibers
Elastic Modulus
Amino Acid Sequence
silk-like protein polymer
silk-elastinlike protein 47K
Brittle fracture
Hydration
Spectrum analysis
Strength of materials
Amino acids
Copolymers
Elastic moduli

ASJC Scopus subject areas

  • Bioengineering
  • Materials Chemistry
  • Polymers and Plastics
  • Biomaterials
  • Medicine(all)

Cite this

Wet-spinning of recombinant silk-elastin-like protein polymer fibers with high tensile strength and high deformability. / Qiu, Weiguo; Teng, Weibing; Cappello, Joseph; Wu, Xiaoyi.

In: Biomacromolecules, Vol. 10, No. 3, 09.03.2009, p. 602-608.

Research output: Contribution to journalArticle

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