Whole-genome comparison of leucine-rich repeat extensins in arabidopsis and rice. A conserved family of cell wall proteins form a vegetative and a reproductive clade

Nicolas Baumberger, Brigitte Doesseger, Romain Guyot, Anouck Diet, Ronald L. Parsons, Mark A. Clark, M. P. Simmons, Patricia Bedinger, Stephen A Goff, Christoph Ringli, Beat Keller

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

We have searched the Arabidopsis and rice (Oryza sativa) genomes for homologs of LRX1, an Arabidopsis gene encoding a novel type of cell wall protein containing a leucine-rich repeat (LRR) and an extensin domain. Eleven and eight LRX (LRR/EXTENSIN) genes have been identified in these two plant species, respectively. The LRX gene family encodes proteins characterized by a short N-terminal domain, a domain with 10 LRRs, a cysteine-rich motif, and a variable C-terminal extensin-like domain. Phylogenetic analysis performed on the conserved domains indicates the existence of two major clades of LRX proteins that arose before the eudicot/monocot divergence and then diversified independently in each lineage. In Arabidopsis, gene expression studies by northern hybridization and promoter::uidA fusions showed that the two phylogenetic clades represent a specialization into "reproductive" and "vegetative" LRXs. The four Arabidopsis genes of the "reproductive" clade are specifically expressed in pollen, whereas the seven "vegetative" genes are predominantly expressed in various sporophytic tissues. This separation into two expression classes is also supported by previous studies on maize (Zea mays) and tomato (Lycopersicon esculentum) LRX homologs and by information on available rice ESTs. The strong conservation of the amino acids responsible for the putative recognition specificity of the LRR domain throughout the family suggests that the LRX proteins interact with similar ligands.

Original languageEnglish (US)
Pages (from-to)1313-1326
Number of pages14
JournalPlant Physiology
Volume131
Issue number3
DOIs
StatePublished - Mar 1 2003
Externally publishedYes

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extensin
Arabidopsis
Leucine
Cell Wall
leucine
cell walls
Genome
rice
genome
Genes
Lycopersicon esculentum
Proteins
genes
proteins
Zea mays
Expressed Sequence Tags
phylogeny
Pollen
Liliopsida
Northern blotting

ASJC Scopus subject areas

  • Plant Science

Cite this

Whole-genome comparison of leucine-rich repeat extensins in arabidopsis and rice. A conserved family of cell wall proteins form a vegetative and a reproductive clade. / Baumberger, Nicolas; Doesseger, Brigitte; Guyot, Romain; Diet, Anouck; Parsons, Ronald L.; Clark, Mark A.; Simmons, M. P.; Bedinger, Patricia; Goff, Stephen A; Ringli, Christoph; Keller, Beat.

In: Plant Physiology, Vol. 131, No. 3, 01.03.2003, p. 1313-1326.

Research output: Contribution to journalArticle

Baumberger, N, Doesseger, B, Guyot, R, Diet, A, Parsons, RL, Clark, MA, Simmons, MP, Bedinger, P, Goff, SA, Ringli, C & Keller, B 2003, 'Whole-genome comparison of leucine-rich repeat extensins in arabidopsis and rice. A conserved family of cell wall proteins form a vegetative and a reproductive clade', Plant Physiology, vol. 131, no. 3, pp. 1313-1326. https://doi.org/10.1104/pp.102.014928
Baumberger, Nicolas ; Doesseger, Brigitte ; Guyot, Romain ; Diet, Anouck ; Parsons, Ronald L. ; Clark, Mark A. ; Simmons, M. P. ; Bedinger, Patricia ; Goff, Stephen A ; Ringli, Christoph ; Keller, Beat. / Whole-genome comparison of leucine-rich repeat extensins in arabidopsis and rice. A conserved family of cell wall proteins form a vegetative and a reproductive clade. In: Plant Physiology. 2003 ; Vol. 131, No. 3. pp. 1313-1326.
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