Whole-transcriptome sequencing identified gene expression signatures associated with aggressive clear cell renal cell carcinoma

Ken Batai, Elliot Imler, Jayce Pangilinan, Robert Bell, Aye Lwin, Elinora Price, Tijana Milinic, Amit Arora, Nathan Ellis, Erika Bracamonte, Bruce Seligmann, Benjamin R. Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Clear cell renal cell carcinoma (ccRCC) is the most prevalent subtype of kidney cancer, yet molecular biomarkers have not been used for the prognosis of ccRCC to aide clinical decision making. This study aimed to identify genes associated with ccRCC aggressiveness and overall survival (OS). Samples of ccRCC tumor tissue were obtained from 33 patients who underwent nephrectomy. Gene expression was determined using whole-transcriptome sequencing. The Cancer Genome Atlas Kidney Renal Clear Cell Carcinoma (TCGA-KIRC) RNA-seq data was used to test association with OS. 290 genes were differentially expressed between tumors with high and low stage, size, grade, and necrosis (SSIGN) score (≥7 vs. ≤3) with PADJ <0.05. Four genes, G6PD, APLP1, GCNT3, and PLPP2, were also over-expressed in advanced stage (III and IV) and high grade (3 and 4) ccRCC and tumor with necrosis (PADJ<0.05). Investigation stratifying by stage found that APLP1 and PLPP2 overexpression were significantly associated with poorer OS in the early stage (Quartile 1 vs. Quartile 4, HR = 3.87, 95% CI:1.25-11.97, P = 0.02 and HR = 4.77, 95% CI:1.37-16.57, P = 0.04 respectively). These genes are potential biomarkers of ccRCC aggressiveness and prognosis that direct clinical and surgical management.

Original languageEnglish (US)
Pages (from-to)247-256
Number of pages10
JournalGenes and Cancer
Volume9
Issue number5-6
DOIs
StatePublished - May 1 2018

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Transcriptome
Renal Cell Carcinoma
Genes
Survival
Neoplasms
Necrosis
Biomarkers
Atlases
Kidney Neoplasms
Nephrectomy
Genome
RNA
Kidney
Gene Expression

Keywords

  • Biomarkers
  • Hispanic americans
  • Kidney cancer
  • Molecular subtype
  • Whole transcriptome sequencing

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

Cite this

Whole-transcriptome sequencing identified gene expression signatures associated with aggressive clear cell renal cell carcinoma. / Batai, Ken; Imler, Elliot; Pangilinan, Jayce; Bell, Robert; Lwin, Aye; Price, Elinora; Milinic, Tijana; Arora, Amit; Ellis, Nathan; Bracamonte, Erika; Seligmann, Bruce; Lee, Benjamin R.

In: Genes and Cancer, Vol. 9, No. 5-6, 01.05.2018, p. 247-256.

Research output: Contribution to journalArticle

Batai, K, Imler, E, Pangilinan, J, Bell, R, Lwin, A, Price, E, Milinic, T, Arora, A, Ellis, N, Bracamonte, E, Seligmann, B & Lee, BR 2018, 'Whole-transcriptome sequencing identified gene expression signatures associated with aggressive clear cell renal cell carcinoma', Genes and Cancer, vol. 9, no. 5-6, pp. 247-256. https://doi.org/10.18632/genesandcancer.183
Batai, Ken ; Imler, Elliot ; Pangilinan, Jayce ; Bell, Robert ; Lwin, Aye ; Price, Elinora ; Milinic, Tijana ; Arora, Amit ; Ellis, Nathan ; Bracamonte, Erika ; Seligmann, Bruce ; Lee, Benjamin R. / Whole-transcriptome sequencing identified gene expression signatures associated with aggressive clear cell renal cell carcinoma. In: Genes and Cancer. 2018 ; Vol. 9, No. 5-6. pp. 247-256.
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