Widespread transcription of a Qa region gene in adult mice

M. J. Palmer, Jeffrey A Frelinger

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The mouse MHC class I family includes genes encoded in four regions: H-2K, H-2D, Qa and Tla. While K/D genes are well characterized, relatively little is known about Qa or Tla genes. We have studied the transcription of a B10.P Qa region gene. DNA sequence comparisons of the transmembrane region, supported by Southern blot analysis of cosmid and genomic DNAs from BALB/c and C57BL/10, demonstrate the λ3a gene corresponds to Q4(p). In both Northern blots and RNA protection experiments using probes derived from the 3' noncoding region, we found that Q4, like the H-2K and H-2D genes, is widely transcribed in B10.P tissues. These data demonstrate for the first time widespread transcription of a Qa gene.

Original languageEnglish (US)
Pages (from-to)95-108
Number of pages14
JournalJournal of Experimental Medicine
Volume166
Issue number1
StatePublished - 1987
Externally publishedYes

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Genes
Cosmids
Southern Blotting
Northern Blotting
RNA
DNA

ASJC Scopus subject areas

  • Immunology

Cite this

Widespread transcription of a Qa region gene in adult mice. / Palmer, M. J.; Frelinger, Jeffrey A.

In: Journal of Experimental Medicine, Vol. 166, No. 1, 1987, p. 95-108.

Research output: Contribution to journalArticle

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