Wire-guided droplet manipulation for molecular biology

Dustin K. Harshman, Jeong-Yeol Yoon

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Wire-guided droplet manipulation (WDM) is a simple method of manipulating liquid droplets in a hydrophobic environment to conduct experiments, reactions, and assays. In WDM, a wire (or needle tip) manipulates microlitersized liquid droplets within an immiscible liquid or on a hydrophobic surface. The attributes of this format for liquid handling address some of the challenges facing the use of conventional techniques. Specifically, WDM provides solutions for development of automated, sample-to-answer, point-of-care systems with potential applications in medicine, life science research, forensics, veterinary diagnostics, and disease control. The widespread applicability of this technique is due to its inherent simplicity stemming from the attractive force between the droplet and the wire. The physics of this interaction will be explained in this chapter. WDM can be applied to standard protocols and is easily reprogrammable for different liquid handling applications. Dilution, mixing, centrifugation, and thermocycling have all been automated by WDM (You and Yoon, J Biol Eng 6:15, 2012). If desired, the principles of droplet manipulation can be easily integrated into the common scientific automation strategy, using commercially available robotic pipetting systems. WDM is automatable, reprogrammable, easy to use, and robust. These are essential features of rapid, all-in-one, sample-to-answer systems to be used at the point-of-care. The applications of WDM within molecular biology that have been demonstrated include DNA extraction (lysing, precipitation, washing, and rehydration), nanoparticle surface deposition for fabrication of a protein nanoarray, immunoassay, PCR thermocycling, and real-time PCR.

Original languageEnglish (US)
Title of host publicationMicrofluidic Methods for Molecular Biology
PublisherSpringer International Publishing
Pages235-252
Number of pages18
ISBN (Electronic)9783319300191
ISBN (Print)9783319300177
DOIs
StatePublished - Jan 1 2016

Keywords

  • Contact angle
  • Electrospinning
  • Electrowetting
  • Endpoint detection
  • Interfacial effects
  • Particle immunoassay
  • PCR inhibition
  • Pendant droplet
  • Polymerase chain reaction (PCR)
  • Protein nanoarray
  • Rapid thermocycling
  • Real-time quantification
  • Sample preparation
  • Sessile droplet
  • Superhydrophobic
  • Water-in-oil emulsion
  • Wire-guided droplet manipulation
  • Work of adhesion

ASJC Scopus subject areas

  • Medicine(all)
  • Immunology and Microbiology(all)
  • Engineering(all)
  • Chemical Engineering(all)

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  • Cite this

    Harshman, D. K., & Yoon, J-Y. (2016). Wire-guided droplet manipulation for molecular biology. In Microfluidic Methods for Molecular Biology (pp. 235-252). Springer International Publishing. https://doi.org/10.1007/978-3-319-30019-1_12