X-RAY ABSORPTION SPECTRA AND THE COORDINATION NUMBER OF Zn AND Co CARBONIC ANHYDRASE AS A FUNCTION OF pH AND INHIBITOR BINDING.

Vittal Yachandra, Linda Powers, Linda S Powers

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

X-ray absorption spectra are reported for native (Zn) and Co-reconstituted bovine carbonic anhydrase (CA), prepared at several pH values across the acid-alkaline transition and in the presence of inhibitors (acetazolamide, imidazole, acetate, HCO//3** minus , NO//3** minus , Cl** minus , CN** minus , OCN** minus , SCN** minus ). The extended fine structure (EXAFS) was analyzed by filtering the first-shell contribution and was compared with a series of model compounds of known structure having various coordination numbers. Co-CA showed variable coordination, as revealed by the calculated number of scatterers and by the average bond distance: four-coordination for the alkaline form and for complexes with CN** minus and acetazolamide, and five-coordination for the acid form and for complexes with acetate and bicarbonate.

Original languageEnglish (US)
Pages (from-to)6596-6604
Number of pages9
JournalJournal of the American Chemical Society
Volume105
Issue number22
StatePublished - Nov 2 1983
Externally publishedYes

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Carbonic anhydrase
Acetazolamide
Carbonic Anhydrases
X ray absorption
X-Rays
Acids
Bicarbonates
Absorption spectra
Acetates
imidazole

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

X-RAY ABSORPTION SPECTRA AND THE COORDINATION NUMBER OF Zn AND Co CARBONIC ANHYDRASE AS A FUNCTION OF pH AND INHIBITOR BINDING. / Yachandra, Vittal; Powers, Linda; Powers, Linda S.

In: Journal of the American Chemical Society, Vol. 105, No. 22, 02.11.1983, p. 6596-6604.

Research output: Contribution to journalArticle

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abstract = "X-ray absorption spectra are reported for native (Zn) and Co-reconstituted bovine carbonic anhydrase (CA), prepared at several pH values across the acid-alkaline transition and in the presence of inhibitors (acetazolamide, imidazole, acetate, HCO//3** minus , NO//3** minus , Cl** minus , CN** minus , OCN** minus , SCN** minus ). The extended fine structure (EXAFS) was analyzed by filtering the first-shell contribution and was compared with a series of model compounds of known structure having various coordination numbers. Co-CA showed variable coordination, as revealed by the calculated number of scatterers and by the average bond distance: four-coordination for the alkaline form and for complexes with CN** minus and acetazolamide, and five-coordination for the acid form and for complexes with acetate and bicarbonate.",
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