Abstract
The homeobox gene koza is a new member of the vertebrate bagpipe-related gene family. Embryonic expression of koza is observed at highest levels in the muscle layer of the somites and, during later development, is restricted to the lateral somitic cells, which correspond to slow twitch muscle tissue. Expression of koza is also observed in the myocardial layer of the heart and in the cement gland. In each of these tissues, koza transcription commences only after the expression of terminal differentiation markers. By injection of synthetic mRNA, we show that overexpression of koza leads to an apparent decrease in the number of cells in the somites. No reduction in cell number is observed when koza is present in neural tissues, suggesting that koza exhibits some tissue specificity in regulation of cell proliferation. Embryonic manipulations show that restriction of koza expression to the slow twitch muscle layer is independent of axial structures but is, at least partly, regulated by signals arising in ectodermal tissue. Finally, in Drosophila, bagpipe expression is regulated by the hedgehog signaling pathway. By using ectopic expression, we show that koza transcription is positively regulated by banded hedgehog. This result indicates that regulation of bagpipe expression by hedgehog signaling is evolutionarily conserved.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 571-580 |
| Number of pages | 10 |
| Journal | Developmental Dynamics |
| Volume | 225 |
| Issue number | 4 |
| DOIs | |
| State | Published - Dec 1 2002 |
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Keywords
- Bagpipe
- Hedgehog signaling
- NK-2 gene
- Somite development
ASJC Scopus subject areas
- Cell Biology
- Developmental Biology
Cite this
Xenopus bagpipe-related gene, koza, may play a role in regulation of cell proliferation. / Newman, Craig S.; Krieg, Paul A.
In: Developmental Dynamics, Vol. 225, No. 4, 01.12.2002, p. 571-580.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Xenopus bagpipe-related gene, koza, may play a role in regulation of cell proliferation
AU - Newman, Craig S.
AU - Krieg, Paul A
PY - 2002/12/1
Y1 - 2002/12/1
N2 - The homeobox gene koza is a new member of the vertebrate bagpipe-related gene family. Embryonic expression of koza is observed at highest levels in the muscle layer of the somites and, during later development, is restricted to the lateral somitic cells, which correspond to slow twitch muscle tissue. Expression of koza is also observed in the myocardial layer of the heart and in the cement gland. In each of these tissues, koza transcription commences only after the expression of terminal differentiation markers. By injection of synthetic mRNA, we show that overexpression of koza leads to an apparent decrease in the number of cells in the somites. No reduction in cell number is observed when koza is present in neural tissues, suggesting that koza exhibits some tissue specificity in regulation of cell proliferation. Embryonic manipulations show that restriction of koza expression to the slow twitch muscle layer is independent of axial structures but is, at least partly, regulated by signals arising in ectodermal tissue. Finally, in Drosophila, bagpipe expression is regulated by the hedgehog signaling pathway. By using ectopic expression, we show that koza transcription is positively regulated by banded hedgehog. This result indicates that regulation of bagpipe expression by hedgehog signaling is evolutionarily conserved.
AB - The homeobox gene koza is a new member of the vertebrate bagpipe-related gene family. Embryonic expression of koza is observed at highest levels in the muscle layer of the somites and, during later development, is restricted to the lateral somitic cells, which correspond to slow twitch muscle tissue. Expression of koza is also observed in the myocardial layer of the heart and in the cement gland. In each of these tissues, koza transcription commences only after the expression of terminal differentiation markers. By injection of synthetic mRNA, we show that overexpression of koza leads to an apparent decrease in the number of cells in the somites. No reduction in cell number is observed when koza is present in neural tissues, suggesting that koza exhibits some tissue specificity in regulation of cell proliferation. Embryonic manipulations show that restriction of koza expression to the slow twitch muscle layer is independent of axial structures but is, at least partly, regulated by signals arising in ectodermal tissue. Finally, in Drosophila, bagpipe expression is regulated by the hedgehog signaling pathway. By using ectopic expression, we show that koza transcription is positively regulated by banded hedgehog. This result indicates that regulation of bagpipe expression by hedgehog signaling is evolutionarily conserved.
KW - Bagpipe
KW - Hedgehog signaling
KW - NK-2 gene
KW - Somite development
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UR - http://www.scopus.com/inward/citedby.url?scp=0036894110&partnerID=8YFLogxK
U2 - 10.1002/dvdy.10186
DO - 10.1002/dvdy.10186
M3 - Article
C2 - 12454933
AN - SCOPUS:0036894110
VL - 225
SP - 571
EP - 580
JO - Developmental Dynamics
JF - Developmental Dynamics
SN - 1058-8388
IS - 4
ER -