ZBP1 enhances cell polarity and reduces chemotaxis

Kyle Lapidus, Jeffrey Wyckoff, Ghassan Mouneimne, Mike Lorenz, Lillian Soon, John S. Condeelis, Robert H. Singer

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The interaction of β-actin mRNA with zipcode-binding protein 1 (ZBP1) is necessary for its localization to the lamellipod of fibroblasts and plays a crucial role in cell polarity and motility. Recently, we have shown that low ZBP1 levels correlate with tumor-cell invasion and metastasis. In order to establish a cause and effect relationship, we expressed ZBP1 in a metastatic rat mammary adenocarcinoma cell line (MTLn3) that has low endogenous ZBP1 levels and delocalized β-actin mRNA. This leads to localization of β-actin mRNA, and eventually reduces the chemotactic potential of the cells as well as their ability to move and orient towards vessels in tumors. To determine how ZBP1 leads to these two apparently contradictory aspects of cell behavior-increased cell motility but decreased chemotaxis-we examined cell motility in detail, both in cell culture and in vivo in tumors. We found that ZBP1 expression resulted in tumor cells with a stable polarized phenotype, and reduced their ability to move in response to a gradient in culture. To connect these results on cultured cells to the reduced metastatic ability of these cells, we used multiphoton imaging in vivo to examine tumor cell behavior in primary tumors. We found that ZBP1 expression actually reduced tumor cell motility and chemotaxis, presumably mediating their decreased metastatic potential by reducing their ability to respond to signals necessary for invasion.

Original languageEnglish (US)
Pages (from-to)3173-3178
Number of pages6
JournalJournal of Cell Science
Volume120
Issue number18
DOIs
StatePublished - Sep 15 2007
Externally publishedYes

Fingerprint

Cell Polarity
Chemotaxis
Carrier Proteins
Aptitude
Cell Movement
Neoplasms
Actins
Messenger RNA
Cultured Cells
Adenocarcinoma
Breast
Cell Culture Techniques
Fibroblasts
Neoplasm Metastasis
Phenotype
Cell Line

Keywords

  • Chemotaxis
  • Intravital imaging
  • Invasion
  • Motility
  • ZBP1

ASJC Scopus subject areas

  • Cell Biology

Cite this

Lapidus, K., Wyckoff, J., Mouneimne, G., Lorenz, M., Soon, L., Condeelis, J. S., & Singer, R. H. (2007). ZBP1 enhances cell polarity and reduces chemotaxis. Journal of Cell Science, 120(18), 3173-3178. https://doi.org/10.1242/jcs.000638

ZBP1 enhances cell polarity and reduces chemotaxis. / Lapidus, Kyle; Wyckoff, Jeffrey; Mouneimne, Ghassan; Lorenz, Mike; Soon, Lillian; Condeelis, John S.; Singer, Robert H.

In: Journal of Cell Science, Vol. 120, No. 18, 15.09.2007, p. 3173-3178.

Research output: Contribution to journalArticle

Lapidus, K, Wyckoff, J, Mouneimne, G, Lorenz, M, Soon, L, Condeelis, JS & Singer, RH 2007, 'ZBP1 enhances cell polarity and reduces chemotaxis', Journal of Cell Science, vol. 120, no. 18, pp. 3173-3178. https://doi.org/10.1242/jcs.000638
Lapidus K, Wyckoff J, Mouneimne G, Lorenz M, Soon L, Condeelis JS et al. ZBP1 enhances cell polarity and reduces chemotaxis. Journal of Cell Science. 2007 Sep 15;120(18):3173-3178. https://doi.org/10.1242/jcs.000638
Lapidus, Kyle ; Wyckoff, Jeffrey ; Mouneimne, Ghassan ; Lorenz, Mike ; Soon, Lillian ; Condeelis, John S. ; Singer, Robert H. / ZBP1 enhances cell polarity and reduces chemotaxis. In: Journal of Cell Science. 2007 ; Vol. 120, No. 18. pp. 3173-3178.
@article{d204f552117e4882bad5f563fb18083c,
title = "ZBP1 enhances cell polarity and reduces chemotaxis",
abstract = "The interaction of β-actin mRNA with zipcode-binding protein 1 (ZBP1) is necessary for its localization to the lamellipod of fibroblasts and plays a crucial role in cell polarity and motility. Recently, we have shown that low ZBP1 levels correlate with tumor-cell invasion and metastasis. In order to establish a cause and effect relationship, we expressed ZBP1 in a metastatic rat mammary adenocarcinoma cell line (MTLn3) that has low endogenous ZBP1 levels and delocalized β-actin mRNA. This leads to localization of β-actin mRNA, and eventually reduces the chemotactic potential of the cells as well as their ability to move and orient towards vessels in tumors. To determine how ZBP1 leads to these two apparently contradictory aspects of cell behavior-increased cell motility but decreased chemotaxis-we examined cell motility in detail, both in cell culture and in vivo in tumors. We found that ZBP1 expression resulted in tumor cells with a stable polarized phenotype, and reduced their ability to move in response to a gradient in culture. To connect these results on cultured cells to the reduced metastatic ability of these cells, we used multiphoton imaging in vivo to examine tumor cell behavior in primary tumors. We found that ZBP1 expression actually reduced tumor cell motility and chemotaxis, presumably mediating their decreased metastatic potential by reducing their ability to respond to signals necessary for invasion.",
keywords = "Chemotaxis, Intravital imaging, Invasion, Motility, ZBP1",
author = "Kyle Lapidus and Jeffrey Wyckoff and Ghassan Mouneimne and Mike Lorenz and Lillian Soon and Condeelis, {John S.} and Singer, {Robert H.}",
year = "2007",
month = "9",
day = "15",
doi = "10.1242/jcs.000638",
language = "English (US)",
volume = "120",
pages = "3173--3178",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "18",

}

TY - JOUR

T1 - ZBP1 enhances cell polarity and reduces chemotaxis

AU - Lapidus, Kyle

AU - Wyckoff, Jeffrey

AU - Mouneimne, Ghassan

AU - Lorenz, Mike

AU - Soon, Lillian

AU - Condeelis, John S.

AU - Singer, Robert H.

PY - 2007/9/15

Y1 - 2007/9/15

N2 - The interaction of β-actin mRNA with zipcode-binding protein 1 (ZBP1) is necessary for its localization to the lamellipod of fibroblasts and plays a crucial role in cell polarity and motility. Recently, we have shown that low ZBP1 levels correlate with tumor-cell invasion and metastasis. In order to establish a cause and effect relationship, we expressed ZBP1 in a metastatic rat mammary adenocarcinoma cell line (MTLn3) that has low endogenous ZBP1 levels and delocalized β-actin mRNA. This leads to localization of β-actin mRNA, and eventually reduces the chemotactic potential of the cells as well as their ability to move and orient towards vessels in tumors. To determine how ZBP1 leads to these two apparently contradictory aspects of cell behavior-increased cell motility but decreased chemotaxis-we examined cell motility in detail, both in cell culture and in vivo in tumors. We found that ZBP1 expression resulted in tumor cells with a stable polarized phenotype, and reduced their ability to move in response to a gradient in culture. To connect these results on cultured cells to the reduced metastatic ability of these cells, we used multiphoton imaging in vivo to examine tumor cell behavior in primary tumors. We found that ZBP1 expression actually reduced tumor cell motility and chemotaxis, presumably mediating their decreased metastatic potential by reducing their ability to respond to signals necessary for invasion.

AB - The interaction of β-actin mRNA with zipcode-binding protein 1 (ZBP1) is necessary for its localization to the lamellipod of fibroblasts and plays a crucial role in cell polarity and motility. Recently, we have shown that low ZBP1 levels correlate with tumor-cell invasion and metastasis. In order to establish a cause and effect relationship, we expressed ZBP1 in a metastatic rat mammary adenocarcinoma cell line (MTLn3) that has low endogenous ZBP1 levels and delocalized β-actin mRNA. This leads to localization of β-actin mRNA, and eventually reduces the chemotactic potential of the cells as well as their ability to move and orient towards vessels in tumors. To determine how ZBP1 leads to these two apparently contradictory aspects of cell behavior-increased cell motility but decreased chemotaxis-we examined cell motility in detail, both in cell culture and in vivo in tumors. We found that ZBP1 expression resulted in tumor cells with a stable polarized phenotype, and reduced their ability to move in response to a gradient in culture. To connect these results on cultured cells to the reduced metastatic ability of these cells, we used multiphoton imaging in vivo to examine tumor cell behavior in primary tumors. We found that ZBP1 expression actually reduced tumor cell motility and chemotaxis, presumably mediating their decreased metastatic potential by reducing their ability to respond to signals necessary for invasion.

KW - Chemotaxis

KW - Intravital imaging

KW - Invasion

KW - Motility

KW - ZBP1

UR - http://www.scopus.com/inward/record.url?scp=35548985713&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35548985713&partnerID=8YFLogxK

U2 - 10.1242/jcs.000638

DO - 10.1242/jcs.000638

M3 - Article

C2 - 17878234

AN - SCOPUS:35548985713

VL - 120

SP - 3173

EP - 3178

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 18

ER -