Zeste maintains repression of Ubx transgenes: Support for a new model of Polycomb repression

Man Wook Hur, Jeffrey D Laney, Sang Hack Jeon, Janann Ali, Mark D. Biggin

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

During late embryogenesis, the expression domains of homeotic genes are maintained by two groups of ubiquitously expressed regulators: the Polycomb repressors and the Trithorax activators. It is not known how the activities of the two maintenance systems are initially targeted to the correct genes. Zeste and GAGA are sequence-specific DNA-binding proteins previously shown to be Trithorax group activators of the homeotic gene Ultrabithorax (Ubx). We demonstrate that Zeste and GAGA DNA-binding sites at the proximal promoter are also required to maintain, but not to initiate, repression of Ubx. Furthermore, the repression mediated by Zeste DNA-binding site is abolished in zeste null embryos. These data imply that Zeste and probably GAGA mediate Polycomb repression. We present a model in which the dual transcriptional activities of Zeste and GAGA are an essential component of the mechanism that chooses which maintenance system is to be targeted to a given promoter.

Original languageEnglish (US)
Pages (from-to)1339-1343
Number of pages5
JournalDevelopment
Volume129
Issue number6
StatePublished - 2002
Externally publishedYes

Fingerprint

Homeobox Genes
Transgenes
Binding Sites
Maintenance
DNA
DNA-Binding Proteins
Embryonic Development
Embryonic Structures
Genes

Keywords

  • Drosophila
  • GAGA
  • Homeotic
  • Polycomb group
  • Trithorax group
  • Zeste

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Hur, M. W., Laney, J. D., Jeon, S. H., Ali, J., & Biggin, M. D. (2002). Zeste maintains repression of Ubx transgenes: Support for a new model of Polycomb repression. Development, 129(6), 1339-1343.

Zeste maintains repression of Ubx transgenes : Support for a new model of Polycomb repression. / Hur, Man Wook; Laney, Jeffrey D; Jeon, Sang Hack; Ali, Janann; Biggin, Mark D.

In: Development, Vol. 129, No. 6, 2002, p. 1339-1343.

Research output: Contribution to journalArticle

Hur, MW, Laney, JD, Jeon, SH, Ali, J & Biggin, MD 2002, 'Zeste maintains repression of Ubx transgenes: Support for a new model of Polycomb repression', Development, vol. 129, no. 6, pp. 1339-1343.
Hur, Man Wook ; Laney, Jeffrey D ; Jeon, Sang Hack ; Ali, Janann ; Biggin, Mark D. / Zeste maintains repression of Ubx transgenes : Support for a new model of Polycomb repression. In: Development. 2002 ; Vol. 129, No. 6. pp. 1339-1343.
@article{f87a171b83c344e99d0bb1892f769d04,
title = "Zeste maintains repression of Ubx transgenes: Support for a new model of Polycomb repression",
abstract = "During late embryogenesis, the expression domains of homeotic genes are maintained by two groups of ubiquitously expressed regulators: the Polycomb repressors and the Trithorax activators. It is not known how the activities of the two maintenance systems are initially targeted to the correct genes. Zeste and GAGA are sequence-specific DNA-binding proteins previously shown to be Trithorax group activators of the homeotic gene Ultrabithorax (Ubx). We demonstrate that Zeste and GAGA DNA-binding sites at the proximal promoter are also required to maintain, but not to initiate, repression of Ubx. Furthermore, the repression mediated by Zeste DNA-binding site is abolished in zeste null embryos. These data imply that Zeste and probably GAGA mediate Polycomb repression. We present a model in which the dual transcriptional activities of Zeste and GAGA are an essential component of the mechanism that chooses which maintenance system is to be targeted to a given promoter.",
keywords = "Drosophila, GAGA, Homeotic, Polycomb group, Trithorax group, Zeste",
author = "Hur, {Man Wook} and Laney, {Jeffrey D} and Jeon, {Sang Hack} and Janann Ali and Biggin, {Mark D.}",
year = "2002",
language = "English (US)",
volume = "129",
pages = "1339--1343",
journal = "Development (Cambridge)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "6",

}

TY - JOUR

T1 - Zeste maintains repression of Ubx transgenes

T2 - Support for a new model of Polycomb repression

AU - Hur, Man Wook

AU - Laney, Jeffrey D

AU - Jeon, Sang Hack

AU - Ali, Janann

AU - Biggin, Mark D.

PY - 2002

Y1 - 2002

N2 - During late embryogenesis, the expression domains of homeotic genes are maintained by two groups of ubiquitously expressed regulators: the Polycomb repressors and the Trithorax activators. It is not known how the activities of the two maintenance systems are initially targeted to the correct genes. Zeste and GAGA are sequence-specific DNA-binding proteins previously shown to be Trithorax group activators of the homeotic gene Ultrabithorax (Ubx). We demonstrate that Zeste and GAGA DNA-binding sites at the proximal promoter are also required to maintain, but not to initiate, repression of Ubx. Furthermore, the repression mediated by Zeste DNA-binding site is abolished in zeste null embryos. These data imply that Zeste and probably GAGA mediate Polycomb repression. We present a model in which the dual transcriptional activities of Zeste and GAGA are an essential component of the mechanism that chooses which maintenance system is to be targeted to a given promoter.

AB - During late embryogenesis, the expression domains of homeotic genes are maintained by two groups of ubiquitously expressed regulators: the Polycomb repressors and the Trithorax activators. It is not known how the activities of the two maintenance systems are initially targeted to the correct genes. Zeste and GAGA are sequence-specific DNA-binding proteins previously shown to be Trithorax group activators of the homeotic gene Ultrabithorax (Ubx). We demonstrate that Zeste and GAGA DNA-binding sites at the proximal promoter are also required to maintain, but not to initiate, repression of Ubx. Furthermore, the repression mediated by Zeste DNA-binding site is abolished in zeste null embryos. These data imply that Zeste and probably GAGA mediate Polycomb repression. We present a model in which the dual transcriptional activities of Zeste and GAGA are an essential component of the mechanism that chooses which maintenance system is to be targeted to a given promoter.

KW - Drosophila

KW - GAGA

KW - Homeotic

KW - Polycomb group

KW - Trithorax group

KW - Zeste

UR - http://www.scopus.com/inward/record.url?scp=0036337188&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036337188&partnerID=8YFLogxK

M3 - Article

C2 - 11880343

AN - SCOPUS:0036337188

VL - 129

SP - 1339

EP - 1343

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

IS - 6

ER -