Zinc enhances adiponectin oligomerization to octadecamers but decreases the rate of disulfide bond formation

David B. Briggs, Rebecca M. Giron, Karina Schnittker, Madeline V. Hart, Chad K. Park, Andrew Hausrath, Tsu Shuen Tsao

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Adiponectin, a hormone secreted from adipocytes, has been shown to protect against development of insulin resistance, ischemia-reperfusion injury, and inflammation. Adiponectin assembles into multiple oligomeric isoforms: trimers, hexamers and several higher molecular weight (HMW) species. Of these, the HMW species are selectively decreased during the onset of type 2 diabetes. Despite the critical role of HMW adiponectin in insulin responsiveness, its assembly process is poorly understood. In this report, we investigated the role of divalent cations in adiponectin assembly. Purified adiponectin 18mers, the largest HMW species, did not collapse to smaller oligomers after treatment with high concentrations of EDTA. However, treatment with EDTA or another chelator DTPA inhibited the oligomerization of 18mers from trimers in vitro. Zn 2+ specifically increased the formation of 18mers when compared with Cu 2+, Mg 2+, and Ca 2+. Distribution of adiponectin oligomers secreted from zinc chelator TPEN-treated rat adipocytes skewed toward increased proportions of hexamers and trimers. While we observed presence of zinc in adiponectin purified from calf serum, the role of zinc in disulfide bonding between oligomers was examined because the process is critical for 18mer assembly. Surprisingly, Zn 2+ inhibited disulfide bond formation early in the oligomerization process. We hypothesize that initial decreases in disulfide formation rates could allow adiponectin subunits to associate before becoming locked in fully oxidized conformations incapable of further oligomerization. These data demonstrate that zinc stimulates oligomerization of HMW adiponectin and possibly other disulfide-dependent protein assembly processes.

Original languageEnglish (US)
Pages (from-to)469-486
Number of pages18
JournalBioMetals
Volume25
Issue number2
DOIs
StatePublished - Apr 2012

Fingerprint

Oligomerization
adiponectin
Adiponectin
disulfide bonds
Disulfides
Zinc
zinc
Molecular weight
Oligomers
Insulin
Ethylenediaminetetraacetic acid
Molecular Weight
molecular weight
sulfides
EDTA (chelating agent)
Chelating Agents
chelating agents
adipocytes
Hormones
Adipocytes

Keywords

  • Adipocyte
  • Adiponectin
  • Adiponectin oligomerization
  • Disulfide bonds
  • Macromolecular protein assembly
  • Zinc

ASJC Scopus subject areas

  • Biomaterials
  • Metals and Alloys
  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Zinc enhances adiponectin oligomerization to octadecamers but decreases the rate of disulfide bond formation. / Briggs, David B.; Giron, Rebecca M.; Schnittker, Karina; Hart, Madeline V.; Park, Chad K.; Hausrath, Andrew; Tsao, Tsu Shuen.

In: BioMetals, Vol. 25, No. 2, 04.2012, p. 469-486.

Research output: Contribution to journalArticle

Briggs, David B. ; Giron, Rebecca M. ; Schnittker, Karina ; Hart, Madeline V. ; Park, Chad K. ; Hausrath, Andrew ; Tsao, Tsu Shuen. / Zinc enhances adiponectin oligomerization to octadecamers but decreases the rate of disulfide bond formation. In: BioMetals. 2012 ; Vol. 25, No. 2. pp. 469-486.
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