Zinc in erythrocyte ghosts

M. Chvapil, David W Montgomery, J. C. Ludwig, C. F. Zukoski

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Proteins and phospholipids but not zinc were released into the medium containing hemoglobin-free ghosts from dog erythrocytes incubated in peroxidation-inducing agents (ascorbic acid, Fe2+,O2). The content of zinc in the pure ghost depends on the purification procedure; using phosphate buffer it amounted to 62 ± 22 μg/g protein, and using barbital buffer-extracted ghost it was three times higher. Although most of the zinc was linked to the lipid phase of the membrane, there is a certain protein moiety of membrane proteins binding more zinc than the other protein fractions. Among various fractions of lipids, zinc is linked mainly to phospholipids and this linkage is not broken by phosphate buffer. It is concluded that zinc is part of the erythrocyte ghost, linked to protein as well as lipid phase.

Original languageEnglish (US)
Pages (from-to)480-484
Number of pages5
JournalProceedings of the Society for Experimental Biology and Medicine
Volume162
Issue number3
StatePublished - 1979

Fingerprint

Erythrocyte Membrane
Zinc
Buffers
Proteins
Lipids
Phospholipids
Phosphates
Barbital
Membrane Lipids
Protein Binding
Ascorbic Acid
Purification
Membrane Proteins
Hemoglobins
Dogs
Membranes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Chvapil, M., Montgomery, D. W., Ludwig, J. C., & Zukoski, C. F. (1979). Zinc in erythrocyte ghosts. Proceedings of the Society for Experimental Biology and Medicine, 162(3), 480-484.

Zinc in erythrocyte ghosts. / Chvapil, M.; Montgomery, David W; Ludwig, J. C.; Zukoski, C. F.

In: Proceedings of the Society for Experimental Biology and Medicine, Vol. 162, No. 3, 1979, p. 480-484.

Research output: Contribution to journalArticle

Chvapil, M, Montgomery, DW, Ludwig, JC & Zukoski, CF 1979, 'Zinc in erythrocyte ghosts', Proceedings of the Society for Experimental Biology and Medicine, vol. 162, no. 3, pp. 480-484.
Chvapil, M. ; Montgomery, David W ; Ludwig, J. C. ; Zukoski, C. F. / Zinc in erythrocyte ghosts. In: Proceedings of the Society for Experimental Biology and Medicine. 1979 ; Vol. 162, No. 3. pp. 480-484.
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