Zuotin, a ribosome-associated DnaJ molecular chaperone

Wei Yan, Brenda Schilke, Christine Pfund, William Walter, Suwon Kim, Elizabeth A. Craig

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

Correct folding of newly synthesized polypeptides is thought to be facilitated by Hsp70 molecular chaperones in conjunction with DnaJ cohort proteins. In Saccharomyces cerevisiae, SSB proteins are ribosome-associated Hsp70s which interact with the newly synthesized nascent polypeptide chain. Here we report that the phenotypes of an S.cerevisiae strain lacking the DnaJ-related protein Zuotin (Zuo1) are very similar to those of a strain lacking Ssb, including sensitivities to low temperatures, certain protein synthesis inhibitors and high osmolarity. Zuo1, which has been shown previously to be a nucleic acid-binding protein, is also a ribosome-associated protein localized predominantly in the cytosol. Analysis of zuo1 deletion and truncation mutants revealed a positive correlation between the ribosome association of Zuo1 and its ability to bind RNA. We propose that Zuo1 binds to ribosomes, in part, by interaction with ribosomal RNA and that Zuo1 functions with Ssb as a chaperone on the ribosome.

Original languageEnglish (US)
Pages (from-to)4809-4817
Number of pages9
JournalEMBO Journal
Volume17
Issue number16
DOIs
StatePublished - Aug 17 1998
Externally publishedYes

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Molecular Chaperones
Ribosomes
HSP40 Heat-Shock Proteins
Proteins
Peptides
Protein Synthesis Inhibitors
Ribosomal RNA
Saccharomyces cerevisiae Proteins
Yeast
Nucleic Acids
Carrier Proteins
Cytosol
Osmolar Concentration
RNA
Saccharomyces cerevisiae
Phenotype
Temperature

Keywords

  • DnaJ
  • Molecular chaperone
  • Ribosome
  • RNA binding
  • Zuo1

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Yan, W., Schilke, B., Pfund, C., Walter, W., Kim, S., & Craig, E. A. (1998). Zuotin, a ribosome-associated DnaJ molecular chaperone. EMBO Journal, 17(16), 4809-4817. https://doi.org/10.1093/emboj/17.16.4809

Zuotin, a ribosome-associated DnaJ molecular chaperone. / Yan, Wei; Schilke, Brenda; Pfund, Christine; Walter, William; Kim, Suwon; Craig, Elizabeth A.

In: EMBO Journal, Vol. 17, No. 16, 17.08.1998, p. 4809-4817.

Research output: Contribution to journalArticle

Yan, W, Schilke, B, Pfund, C, Walter, W, Kim, S & Craig, EA 1998, 'Zuotin, a ribosome-associated DnaJ molecular chaperone', EMBO Journal, vol. 17, no. 16, pp. 4809-4817. https://doi.org/10.1093/emboj/17.16.4809
Yan W, Schilke B, Pfund C, Walter W, Kim S, Craig EA. Zuotin, a ribosome-associated DnaJ molecular chaperone. EMBO Journal. 1998 Aug 17;17(16):4809-4817. https://doi.org/10.1093/emboj/17.16.4809
Yan, Wei ; Schilke, Brenda ; Pfund, Christine ; Walter, William ; Kim, Suwon ; Craig, Elizabeth A. / Zuotin, a ribosome-associated DnaJ molecular chaperone. In: EMBO Journal. 1998 ; Vol. 17, No. 16. pp. 4809-4817.
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